Comprehensive Proteomics Analysis of In Vitro Canine Oviductal Cell-Derived Extracellular Vesicles

Comprehensive Proteomics Analysis of In Vitro Canine Oviductal Cell-Derived Extracellular Vesicles

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Dogs (Canis lupus familiaris) have unique and peculiar reproductive characteristics.While the interplay between in vitro oviductal cell-derived extracellular vesicles (OC-EVs) and cumulus-oocyte complexes in dogs has begun to be elucidated, no study has yet provided extensive Linear Dunes: Morphology from Google Earth information on the biological content and physiological function of OC-EVs and their role in canine oocyte development.Here, we aimed to provide the first comprehensive proteomic analysis of OC-EVs.We identified 398 proteins as present in all OC-EVs samples.The functional enrichment analysis using Gene Ontology terms and an Ingenuity Pathway Analysis revealed that the identified proteins were involved in several cellular metabolic processes, including translation, synthesis, First study on microscopic and molecular detection of Acanthocheilonema reconditum and Leishmania infantum coinfection in dogs in Southwest Colombia expression, and protein metabolism.

Notably, the proteins were also involved in critical canonical pathways with essential functions in oocyte and embryo development, such as ERK/MAPK, EIF2, PI3K/AKT, and mTOR signaling.These data would be an important resource for studying canine reproductive physiology and establishing a successful in vitro embryo production system in dogs.